"Wellness to me means all things in balance. And balance doesn't mean all things are equal or at peace at all times," the 66-year-old who donned a white pantsuit said before losing her footing and falling on the ground.
"Wrong shoes!" she quipped.
A stage assistant rushed to the stage to assist her before she stood up and decided to just ditch her shoes.
"It's nice to be talking about balance and fall," she said.
Among the audience were reportedly Winfrey's best friend Gayle King and Meghan Markle's mother Doria Ragland. Her longtime partner Stedman Graham reportedly attended the Saturday, February 29 motivational speech as well.
Her special guest at the event was Jennifer Lopez joining past guests like Lady GaGa, Dwayne "The Rock" Johnson, and Michelle Obama. Winfrey is scheduled to conclude her "2020 Vision: Your Life on Focus Tour" on March 7 in Denver, Colorado, with her friend Gayle as a guest.
Her sit-down with Lady GaGa in January was an emotional one as the singer opened up on suffering trauma from sexual assault.